\documentclass[12pt,twoside,a4paper]{report}
\usepackage{graphicx} % Required for inserting image
\usepackage{subcaption} % Required for subfigures
\usepackage[T1]{fontenc} %%% for bold small cap
\usepackage{lipsum}  %%%% only for lorem ipsum, can be removed
\graphicspath{{images/}} % to advise LATEX where the graphics are located.
\usepackage{amsmath}  % For the equation* environment
\usepackage[margin=2.5cm]{geometry}  % to adjust margin 
\usepackage{hyperref}   % for hyper references
\usepackage{biblatex} % for bibliograpghy
\addbibresource{Reference.bib}
%%%%%%% Header-Footer %%%%%%%%%%%%%%%
\usepackage{fancyhdr}
\renewcommand{\chaptermark}[1]{\markboth{#1}{}}
\renewcommand{\sectionmark}[1]{\markright{#1}}
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%%%%%%%%% Title,Author,Date    %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\title {This is a basic IISc PhD template using overleaf default font, computer modern}
\author{Harsh Kumar}
\date{July 2024}
%%%%%%%%%%%%%%%%%%%%% Personal details as New commands %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\newcommand{\thesistitle}{Feel Free to Share and Modify: This is a basic IISc PhD template using overleaf default font}
\newcommand{\myname}{Harsh Kumar}
\newcommand{\thesisdate}{July 2024}
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\begin{document}
\pagenumbering{gobble} % Turns off page numbering
\begin{titlepage}
    \centering
     {\huge \textbf{\thesistitle} \par}
      \vspace{2cm}
     {\large \sc A Thesis \par}
      \vspace{0.2cm}
     {\large \sc Submitted For The Degree Of \par}
     \vspace{0.2cm}
      {\large \sc {\textbf{Doctor of Philosophy}} \par} 
     \vspace{0.2cm}
     {\large \sc In The Faculty of Engineering  \par}
     
     \vspace{1cm}
     {\large  by \par}
     \vspace{0.5cm}
     {\large \textbf{\myname} \par}
     \vspace{2cm}
    \includegraphics[width=0.3\textwidth]{Images/IISc_Master_Seal.jpg}\par
    \vspace{1cm}
    {\large \sc Department of Bioengineering \par}
     {\large \sc Indian Institute of Science  \par}
     {\large \sc Bangalore - 560012, India \par}
    \vspace{0.5cm}
    {\large \sc \thesisdate \par}
    \vspace{2cm}
   
\end{titlepage}
%%%%%%%% Blank Page  %%%%%%%%%%%%%%%%
\clearpage
\thispagestyle{empty}
\
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%%%%%%%%%%%%%%%%%%%%%%%% Copyright %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\clearpage
\thispagestyle{empty}
\begin{center}
    \vspace*{\fill}
   \textbf{\textcopyright \ \myname \\ [5pt]
                                \thesisdate\\ [5pt]
                            All rights reserved\\
                            }
    \vspace*{\fill}
\end{center}
\clearpage
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%%%%%%%% Blank Page  %%%%%%%%%%%%%%%%
\clearpage
\thispagestyle{empty}
\
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%%%%%%%%%%%%%%%%%%%%%%%% Dedication page %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\clearpage
\thispagestyle{empty}
\begin{center}
    \vspace*{\fill}
    This thesis is dedicated to\\
    xxxx,xxxx, and xxxx
    \vspace*{\fill}
\end{center}
\clearpage
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%%%%%%%% Blank Page  %%%%%%%%%%%%%%%%
\clearpage
\thispagestyle{empty}
\
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%%%%%%%%%%%%%%%%%%% Acknowledgemnents Page %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\clearpage
\thispagestyle{plain}
\pagenumbering{roman} % Turns off page numbering
\begin{center} 
    \huge\textbf{Acknowledgements}\\
\end{center}
    \normalsize
    I would like to acknowledge everyone in my lab. \lipsum[1-8]
\clearpage
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%%%%%%%%% Contents, Abbreviations, ListofFigures %%%%%%%%%%%%%%%%%%%%%
\tableofcontents %%%%% Table of contents %%%%%%%%
\listoffigures  %%%%% List of figures %%%%%%%%
\listoftables   %%%%%% List of Tables %%%%%%%%%
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
%%%%%%%%%%%%%%%%%%%%%%%%   New Chapter   %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\chapter{Introduction}
\pagenumbering{arabic}
\pagestyle{fancy}
\fancyhf{}
\fancyhead[LE]{\rightmark}
\fancyhead[RO]{\leftmark}
\fancyfoot[C]{\thepage}
\lipsum[1-10]
    %%%%%%%   New Section   %%%%%%%%%
\section{Section 1}
\lipsum[1-3]
\subsection{Subsection Text}
\lipsum[1-5]
\subsection{Subsection Math}
\begin{enumerate}
    \item Sine formula:
    \[
        \sin^2 x + \cos^2 x = 1
    \]
    \item Double-angle formulas:
    \[
        \sin(2x) = 2 \sin x \cos x
    \]
    \[
        \cos(2x) = \cos^2 x - \sin^2 x
    \]
    
    \item Addition formulas:
    \[
        \sin(x + y) = \sin x \cos y + \cos x \sin y
    \]
    \[
        \cos(x + y) = \cos x \cos y - \sin x \sin y
    \]
\end{enumerate}
\subsection{Subsection Text and Figure}
\lipsum[1-3]
 \begin{figure}[htbp]
        \centering
        \begin{subfigure}[b]{0.3\textwidth}
            \centering
            \includegraphics[width=\textwidth]{Images/IISc_Master_Seal.jpg}
            \caption{Image 1}
            \label{fig:1a}
        \end{subfigure}
        \begin{subfigure}[b]{0.3\textwidth}
            \centering
            \includegraphics[width=\textwidth]{Images/IISc_Master_Seal.jpg}
            \caption{Image 2}
             \label{fig:1b}
        \end{subfigure}
        \begin{subfigure}[b]{0.3\textwidth}
            \centering
            \includegraphics[width=\textwidth]{Images/IISc_Master_Seal.jpg}
            \caption{Image 3}
             \label{fig:1c}
        \end{subfigure}
        \caption{Various Images}
    \end{figure}
\lipsum[1-3]\\[2cm]
{\LARGE {Refer figure in a text using label \ref{fig:1b}}}\\ [2cm]
{\LARGE {Refer figure in a text using label \ref{fig:2a}}}\\[2cm]
\lipsum[1-3]
 \begin{figure}[htbp]
        \centering
        \begin{subfigure}[b]{0.45\textwidth}
            \centering
            \includegraphics[width=\textwidth]{Images/IISc_Master_Seal.jpg}
            \caption{First image}
            \label{fig:2a}
        \end{subfigure}
        \begin{subfigure}[b]{0.45\textwidth}
            \centering
            \includegraphics[width=\textwidth]{Images/IISc_Master_Seal.jpg}
            \caption{Second image}
             \label{fig:2b}
        \end{subfigure}
        
        \begin{subfigure}[b]{0.45\textwidth}
            \centering
            \includegraphics[width=\textwidth]{Images/IISc_Master_Seal.jpg}
            \caption{Third image}
             \label{fig:2c}
        \end{subfigure}
        \begin{subfigure}[b]{0.45\textwidth}
            \centering
            \includegraphics[width=\textwidth]{Images/IISc_Master_Seal.jpg} % Replace with the fourth image
            \caption{Fourth image}
             \label{fig:2d}
        \end{subfigure}
        \caption{Four images in two rows}
    \end{figure}
    %%%%%%%   New Section   %%%%%%%%%
\section{Section 2}
\subsection{Subsection A}
\subsection{Subsection B}
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
\chapter{Materials and Methods}
\chapter{Single molecule}
{\large Refer article in text \cite{einstein} }
{\large Refer article in text \cite{Harsh} }
\chapter{Summary and perspectives}
\lipsum[1-10]
\printbibliography
\end{document}